Scaffold integration is studied using various histological techniques. Researchers often use staining methods, such as Hematoxylin and Eosin (H&E) staining, to visualize the scaffold and surrounding tissues. Immunohistochemistry (IHC) can also be employed to detect specific proteins and cellular markers that indicate cell behavior and scaffold integration. Advanced imaging techniques like confocal microscopy and scanning electron microscopy (SEM) provide detailed views of the scaffold structure and cell-scaffold interactions.