RNA analysis is crucial for understanding gene expression patterns in tissues. Common techniques include:
Reverse Transcription PCR (RT-PCR): Converts RNA into complementary DNA (cDNA), which is then amplified and analyzed. In Situ Hybridization (ISH): Uses labeled RNA probes to detect specific RNA molecules within tissue sections, providing spatial context to gene expression. RNA Sequencing (RNA-Seq): Offers a comprehensive view of the transcriptome, allowing for the identification of differentially expressed genes and novel transcripts.
