Rehydration is typically carried out by passing the deparaffinized tissue sections through a graded series of ethanol solutions, from high to low concentrations, followed by rinsing in distilled water. The steps usually include: 1. Immerse slides in 100% ethanol for 1-2 minutes. 2. Transfer slides to 95% ethanol for 1-2 minutes. 3. Follow with 70% ethanol for 1-2 minutes. 4. Rinse slides in distilled water for 1-2 minutes.
