The preparation of polyacrylamide gel involves several steps: 1. Polymerization Reaction: Acrylamide and bisacrylamide are mixed with a catalyst, typically ammonium persulfate (APS), and a stabilizer, such as TEMED. 2. Casting the Gel: The solution is poured into a mold or apparatus, where it polymerizes into a gel matrix. 3. Setting and Running the Gel: After the gel sets, samples are loaded into wells, and an electric field is applied to facilitate the migration of molecules through the gel.
