The preparation of PMMA for embedding involves several steps:
1. Fixation: Tissue samples are first fixed to preserve cellular structures and prevent degradation. 2. Dehydration: The tissue is dehydrated using a series of alcohol solutions to remove water. 3. Infiltration: The dehydrated tissue is infiltrated with a PMMA monomer solution, which replaces alcohol in the tissue spaces. 4. Polymerization: The infiltrated tissue is then placed in a mold and subjected to a polymerization process, usually initiated by a catalyst, to harden the PMMA.
