The application of OCT Compound involves several steps:
1. Preparation: The tissue sample is first prepared by trimming excess material and ensuring it is clean. 2. Embedding: The tissue is placed in a mold, and OCT Compound is added to cover the tissue completely. 3. Freezing: The mold containing the tissue and OCT Compound is rapidly frozen using a cryostat or liquid nitrogen. This process ensures that the tissue and compound form a solid block. 4. Sectioning: Once frozen, the block can be mounted on a cryostat and sectioned into thin slices.
