NADH TR staining involves incubating tissue sections with a solution containing NADH and a tetrazolium salt. The enzyme catalyzes the reduction of tetrazolium to formazan, which precipitates and forms a blue or purple color in areas of high enzymatic activity. The procedure includes several steps: 1. Tissue Preparation: Fresh or frozen tissue sections are preferred for this staining technique. 2. Incubation: The sections are incubated with NADH and tetrazolium salt under controlled conditions. 3. Observation: The stained sections are examined under a light microscope to visualize the formazan deposits.
