Performing micro indentation involves several steps:
1. Sample Preparation: Tissue samples need to be properly prepared, often by fixing, embedding, and sectioning them into thin slices. 2. Indentation Process: A micro indenter, typically equipped with a diamond or hard metal tip, is pressed into the tissue sample with a known force. 3. Data Collection: The depth of indentation and the applied force are recorded, providing data on the tissue's mechanical properties. 4. Data Analysis: The collected data is analyzed to determine various mechanical parameters, such as hardness and elastic modulus.
