How is India Ink Applied in Histological Procedures?
India ink is typically applied directly to the surface of fresh tissue specimens. The technique involves gently painting the ink onto the tissue with a fine brush or applicator. Once applied, the ink is allowed to dry before the tissue is processed for further histological examination. The carbon particles in the ink adhere to the tissue surface, providing a persistent mark that remains visible throughout the fixation, embedding, and sectioning processes.
