The process typically involves the following steps:
Preparing the tissue sample: The sample is often embedded in a medium such as paraffin and sectioned. Placing the sample under the indenter: The sample is positioned on a stage, and the indenter is aligned with the area of interest. Applying force: A controlled force is applied using the indenter, which is often a diamond tip. Measuring deformation: The deformation caused by the indenter is measured to determine mechanical properties like hardness and elasticity.
