The process of in vivo stimulation involves several steps:
Selection of the Model Organism: Commonly used organisms include mice, rats, and zebrafish, chosen based on the specific research question and the biological systems involved. Application of Stimuli: This can range from chemical agents, such as drugs or toxins, to physical stimuli like electrical impulses or mechanical stress. The method of application can vary, including oral administration, injection, or localized delivery. Observation and Analysis: Following stimulation, tissues are often harvested and subjected to various histological techniques, such as staining, immunohistochemistry, or electron microscopy, to analyze the effects.
