1. Oocyte Retrieval: Mature oocytes are collected from the female partner through a process known as transvaginal ultrasound aspiration. 2. Sperm Preparation: Sperm is collected from the male partner and processed to select the most motile and morphologically normal sperm. 3. Micromanipulation: Under a high-powered microscope, a single sperm is picked up using a fine glass needle called a micropipette. 4. Injection: The sperm is then directly injected into the cytoplasm of the oocyte. 5. Fertilization and Embryo Culture: The injected oocyte is cultured in an incubator to monitor fertilization and embryo development.
