How is Hydrogen Peroxide Applied in Histological Procedures?
Hydrogen peroxide can be applied to tissue sections through various methods, depending on the specific requirements of the protocol:
1. Pre-treatment: Before staining, tissue sections can be pre-treated with hydrogen peroxide to block endogenous peroxidase activity. Typically, a solution of 0.3% hydrogen peroxide in methanol or phosphate-buffered saline (PBS) is used.
2. Staining Protocols: During staining procedures, hydrogen peroxide is often included in the staining solution. For example, in the DAB staining protocol, hydrogen peroxide is mixed with DAB and applied to the tissue sections.
