The process of gene knockout can be achieved using several techniques. The most common methods include CRISPR-Cas9 and homologous recombination. CRISPR-Cas9 involves the use of a guide RNA to target and cut the DNA at a specific location, resulting in the disruption of the gene. Homologous recombination, on the other hand, involves the insertion of a DNA sequence that disrupts the gene, often by replacing it with a selectable marker.
