FITC conjugation involves attaching the FITC molecule to an antibody or other protein. The process typically includes:
Activation: The protein of interest is activated, often through the introduction of amine groups. Reaction: The activated protein is mixed with FITC under controlled conditions, allowing the isothiocyanate group of FITC to form a stable covalent bond with the protein. Purification: The FITC-conjugated protein is purified to remove any unbound FITC, ensuring specificity in subsequent applications.
