enzyme purity

How is Enzyme Purity Measured?

Several methods are used to measure enzyme purity, including:
Electrophoresis: This technique separates proteins based on their size and charge, allowing for the detection of contaminants.
Chromatography: Techniques such as ion-exchange, gel-filtration, and affinity chromatography are used to separate and purify enzymes.
Spectrophotometry: Measures the enzyme's activity and compares it to known standards to determine purity.
Mass spectrometry: Provides detailed information about the molecular weight and structure of the enzyme, identifying contaminants.

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