Several methods are used to measure enzyme purity, including:
Electrophoresis: This technique separates proteins based on their size and charge, allowing for the detection of contaminants. Chromatography: Techniques such as ion-exchange, gel-filtration, and affinity chromatography are used to separate and purify enzymes. Spectrophotometry: Measures the enzyme's activity and compares it to known standards to determine purity. Mass spectrometry: Provides detailed information about the molecular weight and structure of the enzyme, identifying contaminants.