Enzyme activity can be measured using various techniques:
1. Histochemical Staining: This method uses enzyme substrates that produce a colored product upon reaction. For example, the enzyme alkaline phosphatase reacts with specific substrates to produce a visible color in tissue sections. 2. Spectrophotometry: This technique measures the absorbance of light by the reaction product, allowing quantification of enzyme activity. 3. Fluorometry: Similar to spectrophotometry but uses fluorescent substrates, which upon reaction emit light that can be measured.
