Fixation: The tissue is fixed in a chemical solution to preserve cellular components and morphology. Dehydration: The tissue is dehydrated through a series of alcohol solutions to remove water content. Clearing: The dehydrated tissue is cleared using a solvent like xylene to make it transparent. Infiltration: The tissue is infiltrated with the embedding medium, often under heat or vacuum conditions. Embedding: The infiltrated tissue is positioned in a mold and allowed to solidify.
