ECM protein coatings are typically applied to culture surfaces, such as glass slides or tissue culture plates. The general steps involve:
Surface preparation: Cleaning and sterilizing the substrate. Solution preparation: Diluting the ECM protein (e.g., collagen) in a suitable buffer. Coating process: Applying the solution to the substrate and allowing it to dry or incubate. Rinsing: Removing excess protein by washing with a buffer.