In histology, the study of E2 enzymes involves the use of various techniques to visualize and quantify their presence and activity in tissue samples. Immunohistochemistry (IHC) is commonly employed to detect E2 enzymes using specific antibodies. This method allows researchers to observe the localization and expression levels of E2 enzymes within different cell types and tissues. Western blotting and immunofluorescence are also used to complement histological analyses by providing additional data on protein expression and interaction.