The process of DNA digestion typically involves the following steps:
Selection of Enzyme: Choose a specific restriction enzyme or nuclease based on the target DNA sequence. Preparation of Reaction Mixture: Combine the DNA sample with the enzyme, buffer, and other necessary components. Incubation: Allow the reaction mixture to incubate at the optimal temperature for the enzyme's activity. Stopping the Reaction: Use heat or chemical methods to terminate the enzyme activity, preventing further digestion.
