How is Data Analyzed in Multiplexed Immunofluorescence?
Data analysis in mIF involves several steps:
- Image Acquisition: High-resolution images are captured using a fluorescence microscope. - Image Processing: Software tools are used to correct for background fluorescence, deconvolve overlapping signals, and enhance image quality. - Quantification: Fluorescence intensity and distribution are quantified using image analysis software, providing data on protein expression levels and colocalization. - Statistical Analysis: The quantitative data are subjected to statistical analysis to identify significant differences and correlations between biomarkers.
