How is Cytochrome c Detected in Histological Studies?
Cytochrome c can be detected using various histological techniques: - Immunohistochemistry (IHC): This technique uses antibodies specific to cytochrome c to visualize its location within cells and tissues. The presence of cytochrome c in the cytoplasm rather than the mitochondria can indicate apoptotic activity. - Western Blotting: Although not strictly a histological technique, Western blotting can be used to quantify cytochrome c levels in cell lysates, providing additional information on its role in apoptosis or electron transport.
