Tissue Collection: Fresh tissue samples are collected and kept on ice to prevent degradation. Embedding Medium: The tissue is placed in an embedding medium, usually OCT compound (Optimal Cutting Temperature compound), which supports the tissue during freezing and sectioning. Freezing: The tissue embedded in OCT compound is rapidly frozen using a cryostat or liquid nitrogen. Rapid freezing prevents the formation of ice crystals that can damage cellular structures. Sectioning: The frozen tissue is sectioned using a cryostat, a specialized microtome for cutting thin sections of frozen tissue. Staining: The tissue sections are stained using various histological stains to highlight different cellular components and structures.
