Sample Preparation: The sample must be prepared to be compatible with multiple imaging modalities. This can involve staining, fixation, and embedding techniques. Initial Imaging: Typically, the sample is first imaged using a lower-resolution technique like light microscopy to identify regions of interest. High-Resolution Imaging: The identified regions are then subjected to higher-resolution techniques such as electron microscopy. Specific markers or fluorescent dyes can be used to highlight structures or functions. Data Correlation: Images from different techniques are aligned and correlated using specialized software to create a comprehensive dataset.
