How is Colloidal Gold Prepared for Histological Use?
Preparation of colloidal gold for histological applications involves several steps: 1. Synthesis: Gold nanoparticles are synthesized using methods such as citrate reduction, where gold salts are reduced to form nanoparticles. 2. Functionalization: The synthesized nanoparticles are functionalized with linker molecules to attach antibodies or other targeting molecules. 3. Purification: The conjugated nanoparticles are purified to remove unbound molecules and ensure specificity in labeling.
