In Histology, cellular injury can be detected using various staining techniques and microscopic examinations. Some common methods include:
Hematoxylin and Eosin Staining (H&E): Reveals changes in cell morphology, such as swelling, nuclear alterations, and cytoplasmic changes. Special Stains: Techniques like Periodic Acid-Schiff (PAS) and Oil Red O can identify specific cellular components and changes. Immunohistochemistry (IHC): Uses antibodies to detect specific proteins involved in cellular injury and apoptosis. Electron Microscopy: Provides detailed images of cellular ultrastructure, revealing mitochondrial damage, membrane disruptions, and other subcellular changes.
