During the preparation of tissue sections for immunohistochemical staining, a blocking solution containing BSA is applied to the tissue. Typically, a 1-5% BSA solution in phosphate-buffered saline (PBS) is used. The tissue sections are incubated with this solution for a period, usually ranging from 30 minutes to an hour. This step is crucial in minimizing non-specific interactions and improving the signal-to-noise ratio in the final stained images.
