How is ATP Depletion Detected in Histological Samples?

Detecting ATP depletion in histological samples involves a combination of techniques:

- Staining Methods: Specific stains like H&E (Hematoxylin and Eosin) can reveal morphological changes such as cell swelling and membrane damage.
- Immunohistochemistry: This technique can be used to detect the expression levels of ATP-dependent enzymes or proteins that are affected by ATP depletion.
- Electron Microscopy: Provides detailed images of cellular ultrastructure, revealing mitochondrial damage and other subcellular alterations due to ATP depletion.