The ATP assay typically involves the use of a luciferase enzyme that catalyzes the reaction between ATP and luciferin, producing light. The amount of light emitted is proportional to the ATP concentration in the sample. The steps generally include:
1. Sample Preparation: Tissue samples are homogenized and lysed to release ATP. 2. Reagent Addition: A luciferase-luciferin reagent is added to the sample. 3. Luminescence Measurement: The light emitted from the reaction is measured using a luminometer or a similar device.
