1. Preparation: Dissolving agarose powder in buffer and pouring it into a casting tray. 2. Loading Samples: Once the gel solidifies, samples mixed with a loading dye are loaded into wells. 3. Running the Gel: An electric field is applied, causing negatively charged nucleic acids to migrate towards the positive electrode. 4. Visualization: After electrophoresis, the gel is stained with a dye such as ethidium bromide or SYBR Green, allowing visualization of the separated bands under UV light.
