The required time for each histological step is determined by various factors, including the type and size of the tissue, the fixative used, and the desired staining technique. For example, smaller tissues may require less fixation time compared to larger samples. The type of fixative, such as formalin or glutaraldehyde, also influences the duration needed. Protocols and guidelines provided in histology manuals and research papers can offer a standardized approach to determining adequate time.
