The polymerization of acrylamide is initiated by free radicals. Common initiators include ammonium persulfate (APS) and tetramethylethylenediamine (TEMED). The concentration of these initiators, along with the concentration of acrylamide and bisacrylamide, can be adjusted to control the polymerization rate and the properties of the resulting gel. The polymerization process is also temperature-sensitive, with higher temperatures accelerating the reaction.