The creation of a paraffin block involves several steps:
1. Fixation: The tissue sample is fixed using chemicals like formalin to preserve it and prevent degradation. 2. Dehydration: The fixed tissue is dehydrated through a series of increasing concentrations of alcohol to remove water. 3. Clearing: Alcohol is replaced with a clearing agent like xylene, making the tissue transparent. 4. Infiltration: The cleared tissue is infiltrated with melted paraffin wax. 5. Embedding: The tissue infiltrated with paraffin is placed in a mold with more melted paraffin and allowed to solidify.
