The process of achieving 3D visualization typically involves the following steps:
Sample Preparation: Tissues are fixed, embedded, and sectioned into thin slices. Imaging: Sections are imaged using techniques such as confocal microscopy, optical coherence tomography (OCT), or micro-CT scanning. Image Reconstruction: Specialized software is used to stack and align the 2D images to create a 3D model. Visualization and Analysis: The 3D model can be manipulated, rotated, and analyzed to study the tissue in detail.