Wortmannin exerts its effects by binding to the catalytic subunit of PI3Ks, thereby inhibiting their activity. This inhibition disrupts the conversion of phosphatidylinositol (4,5)-bisphosphate to phosphatidylinositol (3,4,5)-trisphosphate, a critical step in the PI3K/Akt signaling pathway. This pathway is vital for regulating cell growth, proliferation, survival, and metabolism.
