Staining is critical in histology as it enhances the contrast of structures within tissues. However, the choice of stain and its application can lead to variability in staining results. For example, Hematoxylin and Eosin (H&E) staining is widely used, but different batches of stains or variations in staining protocols can result in differences in tissue coloration. Special stains, such as Masson's Trichrome or Periodic acid-Schiff (PAS), can also produce variable results depending on the technique used.
