Grimelius staining involves the use of silver nitrate, which binds to reducing substances in the cells. The procedure typically includes the following steps:
Fixation of tissue samples in formalin or another suitable fixative. Embedding the tissue in paraffin and cutting it into thin sections. Deparaffinization and rehydration of the tissue sections. Incubation with a silver nitrate solution, which is reduced to form visible silver deposits by the argentaffin granules in the cells. Counterstaining to enhance the contrast between stained and unstained tissue components.
