darkfield illumination

How Does Darkfield Illumination Work?

In darkfield microscopy, a specialized condenser directs light at a sharp angle towards the specimen. The majority of the light misses the objective lens, resulting in a dark background. Only the light scattered by structures within the specimen enters the objective lens, creating a bright image of the structures against the dark background. This method is particularly useful for observing fine details and structures that are otherwise invisible under brightfield illumination.

Frequently asked queries:

Partnered Content Networks

Relevant Topics