The debranching enzyme has two distinct activities: a 4-α-glucanotransferase activity and an amylo-1,6-glucosidase activity. The 4-α-glucanotransferase activity transfers a block of three glucose residues from a branch to a nearby linear chain, while the amylo-1,6-glucosidase activity hydrolyzes the remaining single glucose residue at the branch point. This dual functionality ensures the complete breakdown of glycogen molecules into glucose units.
