How Do You Determine the Appropriate Excitation and Emission Wavelengths?
First, identify the light source and filters available in your fluorescence microscope. Most microscopes have specific filter sets designed for common fluorophores. Consult the spectral properties of the fluorophores to ensure they match your equipment. For example, if you are using a blue laser, you would need a fluorophore with an excitation peak around 488 nm.
