Tight junction proteins interact through a complex network of protein-protein interactions. Claudins form strands that create the primary barrier to paracellular transport. Occludin and JAMs provide additional support and regulation to the tight junction assembly. These proteins are anchored to the actin cytoskeleton through interactions with cytoplasmic plaque proteins such as ZO-1, ZO-2, and ZO-3, which are part of the MAGUK family of proteins.
