Histologists use several methods to study protein structure within tissues.
Immunohistochemistry (IHC) IHC involves the use of antibodies to detect specific proteins in tissue sections. The antibody binds to its target protein, and the complex can be visualized using various detection methods, often involving chromogenic or fluorescent labels.
Western Blotting Western blotting is used to detect and quantify specific proteins extracted from cells or tissues. Proteins are separated by gel electrophoresis and then transferred to a membrane, where they are probed with specific antibodies.
Mass Spectrometry Mass spectrometry provides detailed information about protein structure, including the identification of post-translational modifications. It is often used in conjunction with other techniques to provide a comprehensive understanding of protein structure and function.
