Histochemical techniques typically involve the following steps:
Fixation: Preserving the tissue sample to prevent degradation and maintain cellular structure. Common fixatives include formaldehyde and glutaraldehyde. Embedding: The tissue is embedded in a medium, usually paraffin wax, to provide support for thin sectioning. Sectioning: Thin slices of the embedded tissue are cut using a microtome, allowing for better staining and visualization. Staining: Application of specific stains to highlight different cellular components. Visualization: Examination of the stained tissue under a microscope to identify and analyze cellular structures.
