How Do Chemical Reactions Aid in Histological Techniques?
Many histological techniques involve chemical reactions that help visualize specific tissue components. For instance, the Periodic Acid-Schiff (PAS) reaction is used to detect polysaccharides such as glycogen in tissues. This reaction involves the oxidation of polysaccharides by periodic acid, followed by the formation of a magenta-colored complex with Schiff reagent. Such chemical reactions are essential for identifying and studying various biochemical constituents of tissues.