How Do 3D Techniques Differ from Traditional 2D Histology?

Traditional 2D histology involves sectioning tissues into thin slices and examining them under a microscope. While effective for many applications, this approach can miss crucial spatial information and the three-dimensional structure of tissues. In contrast, 3D techniques provide a more comprehensive view, capturing the entire volume of the tissue. This allows for the study of tissue morphology and cellular interactions in their native state, offering a more accurate representation of biological processes.