How Can Macropinocytosis Be Studied in the Laboratory?
Laboratory techniques to study macropinocytosis include the use of fluorescently labeled dextrans or other macromolecules that are internalized by cells through this pathway. Live cell imaging can track the formation and maturation of macropinosomes in real-time. Additionally, genetic and pharmacological manipulation of key regulatory pathways can help elucidate the molecular mechanisms underlying macropinocytosis. Immunohistochemistry and electron microscopy provide detailed views of macropinosomes and their interactions with other cellular structures.
