How Are Vascular Changes Identified Histologically?
Vascular changes are identified using various histological techniques. Common methods include:
1. Hematoxylin and Eosin (H&E) Staining H&E staining is the most widely used technique in histology. It helps differentiate between cellular and extracellular components, allowing for the visualization of structural changes in blood vessels.
2. Immunohistochemistry This technique uses antibodies to detect specific proteins within the tissue. For instance, markers such as von Willebrand factor can be used to identify endothelial cells, while smooth muscle actin can highlight changes in smooth muscle cells.
3. Electron Microscopy Electron microscopy provides detailed images of cellular and subcellular structures. It is particularly useful in identifying ultrastructural changes in endothelial cells and the ECM.
