The production of synthetic antibodies involves several steps:
Gene Synthesis: Designing and synthesizing the DNA sequences that encode the antibodyâs variable regions. Vector Cloning: Inserting the synthetic genes into a suitable expression vector. Transfection: Introducing the vector into host cells, commonly CHO cells (Chinese Hamster Ovary cells) or HEK293 cells (Human Embryonic Kidney cells). Expression and Purification: Culturing the host cells to produce the antibody, followed by purification using techniques like affinity chromatography.
