The process of applying stains typically involves several steps:
1. Fixation: Tissues are preserved using chemicals like formalin to prevent degradation. 2. Embedding: The fixed tissues are embedded in a solid medium, usually paraffin wax, to facilitate sectioning. 3. Sectioning: Thin slices of the embedded tissue are cut using a microtome. 4. Staining: The tissue sections are treated with various stains according to the desired outcome. 5. Mounting: The stained sections are mounted on glass slides for examination under a microscope.
